Figure 8. Increased miR-192-5p levels in ischemic muscles. Skeletal muscle samples were harvested from the ischemic limb of patients undergoing above the knee amputations for CLI. Tibialis anterior and sartorious muscle samples were harvested and total RNA was extracted. The bar graph shows average ±SEM miR-192-5p levels measured by qPCR. Values of individual patients are indicated as black squares. For each individual, ischemic sartorius muscle values are referred to non-ischemic sartorius values (n= 7; **p< 0.01).