Figure 4. Effects of chronic exercise on hypothalamic TGF-β1 protein levels in Middle-Aged obese mice. Western blotting of IkB-α ser32 phosphorylation (A) and TGF-β1 protein level (C) and Protein level normalization with Tubulin (right) (B and D) (n= 8 per group). All analyses were made after the last day of training. Data are expressed as means ± SEM. *, p<0.05 vs Sedentary group.