Research Paper Volume 9, Issue 5 pp 1359—1374

Figure 8. Neutrophils inhibit LPC response through oxidative stress and induce DNA damage during aging. (A) EpCAM(red)/γ-H2A.X(green)/DAPI(blue) staining of liver tissues from Y/O mice with normal/CDE diet. Numbers of EpCAM⁺γ-H2A.X⁺ cells were quantified. Results are mean ± SEM from three independent experiments (n > 3 mice per group). (B) Old mice with CDE diet were subjected to NAC drinking (1 g/L). Cumulative survival rates of mice were analyzed. n > 5 mice per group. (C) Representative FACS scatterplots and summarized percentages of EpCAM⁺CD45^- cells in liver tissues from O-CDE mice treated with NAC drinking. Results are mean ± SEM from three independent experiments (n > 3 mice per group). (D) EpCAM(red)/γ-H2A.X(green)/DAPI(blue) staining of liver tissues from O-CDE mice treated with NAC drinking. Numbers of EpCAM⁺γ-H2A.X⁺ cells were quantified. Results are mean ± SEM from three independent experiments (n > 6 mice per group). (E) Representative FACS scatterplots of liver-infiltrating neutrophils (CD11b⁺Gr-1^high) in liver tissues from O-CDE mice treated with NAC drinking. (F) Hepatic lipid peroxidation was measured by MDA assay. Results are mean ± SEM from three independent experiments (n > 6 mice per group). *P < 0.05, **P < 0.01.