Research Paper Volume 9, Issue 5 pp 1359—1374

Figure 6. Hepatic stellate cell-derived CXCL7 induced neutrophil infiltration into livers of O-CDE mice. (A) mRNA levels of CXCL1/CXCL7 in livers from Y/O mice with normal/CDE diet were measured by Q-PCR. Results are mean ± SEM from three independent experiments (n = 3 mice per group). (B) PCR analysis of CXCL7 expression in hepatic stellate cells and leukocytes isolated from livers of Y/O-CDE mice. (C) mRNA level of CXCL7 in hepatic stellate cells and leukocytes isolated from Y/O-CDE mice with CDE diet were measured by Q-PCR. Results are mean ± SEM from three independent experiments (n = 3 mice per group). (D) Levels of CXCR2 expression on neutrophils derived from livers were analyzed by FCM. Fluorescence intensity of CXCR2 was analyzed. Results are mean ± SEM from three independent experiments (n > 3 mice per group). (E) Mobility of neutrophils in response to conditioned medium from Y/O-CDE hepatic stellate cells was analyzed in the absence or presence of SB225002 (100 nM). Numbers of migrated neutrophils were determined. Results are mean ± SEM from three independent experiments (n = 3 per group). *P < 0.05, **P < 0.01. (F) Quantification of liver-infiltrating neutrophils (CD11b⁺Gr-1^high) from Y/O mice with normal/CDE diet treated with SB225002 (2 mg/kg) or control by FCM. Results are mean ± SEM from three independent experiments (n > 4 mice per group).