Research Paper Volume 9, Issue 5 pp 1359—1374

Figure 2. LPC activation and liver regeneration are impaired in old mice. Y/O mice were fed with normal/CDE diet for 3 weeks. At day 21, liver tissues from Y/O mice were collected and analyzed. (A) mRNA levels of EpCAM, CD133 and AFP in livers were measured by Q-PCR. Results are mean ± SEM from three independent experiments (n > 6 mice per group). (B) EpCAM⁺CD45^- cells in NPCs from Y/O mice with normal/CDE diet were analyzed by FCM. Percentage of EpCAM⁺CD45^- cells was quantified. Results are mean ± SEM from three independent experiments (n > 3 mice per group). (C) EpCAM(red)/Ki67(green)/DAPI(blue) staining of liver tissues. a-d, scale bar = 100 μm; e-h, scale bar = 20 μm. Numbers of EpCAM⁺ and EpCAM⁺Ki67⁺ cells were quantified. Results are mean ± SEM from three independent experiments (n > 3 mice per group). (D) mRNA level of cyclin E1 in EpCAM⁺CD45^- cells from Y/O mice with normal/CDE diet was measured by Q-PCR. Results are mean ± SEM from three independent experiments (n > 6 mice per group). *P < 0.05, **P < 0.01.