Research Paper Volume 9, Issue 4 pp 1233—1247

Figure 6. Aspirin sensitized TMZ therapy. (A) U87 and T98G cells were treated with aspirin (1 mM) alone, TMZ (100 µM) alone or both for 72 h. Cell survival was analyzed using CCK-8 assay. (B) U87 and T98G cells were treated by aspirin (1 mM) alone, TMZ (100 µM) alone or both for 72 h. Western blot analysis showed GLI1, Foxm1, p-p65, p65, γH2AX, p-ATM, ATM, N-GLI1 and Sufu proteins expression. β-actin and Histone H3 were used as an internal control. T98G and U87 cells were treated with aspirin (1 mM) alone, TMZ (100 µM) alone or both for 72 h and then collected for comet assay (C) and immunofluorescence (D) respectively. (E) TMZ-induced upregulation of MGMT in T98G cells after exposed to the TMZ (100 µM) with or without aspirin (1 mM) were assayed at the end of each time-point by Western blot. Data are shown as mean±SEM for the three replicates. Statistical significance levels are indicated as: *P < 0.05; **P < 0.01 and ***P < 0.001.