Figure 10A-F. Effect of MSDK on RUNX2, β1 integrin, NFκB and metabolic proteins. After 21 days of MSDK treatment, western blot was performed to determine (A) RUNX2 expression of osteoblasts grown in transwell co-cultures, (B) RUNX2 expression of osteoblasts grown in layered co-culture, (C) β1 integrin expression of osteoblasts grown in transwell co-cultures, (D) β1 integrin expression of osteoblasts and osteoclasts grown in layered co-cultures (E) NFκB expression of osteoclasts grown in transwell co-cultures, and (F) NFκB expression of osteoblasts and osteoclasts grown in layered co-cultures.