Calcium alterations signal either to senescence or to autophagy induction in stem cells upon oxidative stress

Aleksandra V. Borodkina; Alla N. Shatrova; Pavel I. Deryabin; Anastasiia A. Griukova; Polina A. Abushik; Sergei M. Antonov; Nikolay N. Nikolsky; Elena B. Burova

doi:doi:10.18632/aging.101130

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Research Paper Volume 8, Issue 12 pp 3400—3418

Calcium alterations signal either to senescence or to autophagy induction in stem cells upon oxidative stress

Figure 6. BAPTA induces an AMPK-dependent autophagy in H₂O₂-treated hMESCs. Cells were treated as indicated in the legend to Figure 3 and were subsequently analyzed by western blotting with the indicated antibodies. (a) Western blot analysis of pAMPK, pTSC2, p70S6K and p4E-BP1 at the various time points after H₂O₂ addition. (b) Alterations in ULK1 phosphorylation at Ser757 and Ser555 as well as LC3 lipidization induced by BAPTA pretreatment in H₂O₂-stimulated cells. Representative results of the three experiments are shown in the Figure. GAPDH was used as loading control.