Research Paper Volume 8, Issue 12 pp 3400—3418

Figure 5. BAPTA attenuates activation of both DNA damage response and p53/p21/Rb pathway in H₂O₂-treated hMESCs. Cells were treated as indicated in the legend to Figure 3 and were subsequently analyzed by western blotting with the indicated antibodies at the various time points. (a) Phosphorylation levels of the main DDR members: ATM, H2A.X, 53BP1, as well as p53. (b) Western blot analysis of p53 and Rb phosphorylation, and p21 protein expression performed at indicated time points. Representative results of the three experiments are shown in the Figure. GAPDH was used as loading control.