Research Paper Volume 8, Issue 12 pp 3400—3418

Figure 3. Loading cells with BAPTA effectively decreases H₂O₂-induced [Ca²⁺]_i elevation, and has no effect on cell viability. Cells were either pretreated or not with 10 µM BAPTA (loading procedure is described in “Materials and Methods” section), then were subjected to 200 µM H₂O₂ for 1 h with the following H₂O₂ replacement and cell cultivation under normal conditions for the indicated time. (a) Intracellular calcium levels measured by FACS after staining hMESCs with the fluorescent probe Fluo-3AM at day 6 after the oxidative stress. (b) Application of 10 µM BAPTA had no effect on viability of H₂O₂-treated hMESCs. The percentage of viable cells was evaluated in 24 h after H₂O₂ treatment by FACS analysis as described in “Materials and Methods” section. M ± Std.dev., N=3. **p˂0.005, versus control; §§p˂0.005, versus H₂O₂-treated cells by Student’s t-test. Ctr – untreated cells.