Research Paper Volume 8, Issue 11 pp 3028—3044

Figure 4. dfoxo partially mediates effects on longevity and stress resistance observed in male rpd3 mutant flies. (A, B) rpd3 (A) and dfoxo (B) mRNA levels in the heads and thoraces of rpd3^def/yw, rpd3^def/dfoxo^c01841and dfoxo^c01841/yw male flies at 40 days determined by qPCR (n=3, 30 Heads and thoraces per replicate. p as noted, t test, error bars represent SEM). (C,D) Survival curves of male (C) and female (D) rpd3^def/yw, rpd3^def/dfoxo^c01841 and dfoxo^c01841/yw flies. (E-F) Survival curves of rpd3^def/+ and control flies male and female flies on 5% H₂O₂ at age 10 (E) and 40 (F) days. (G) Survival curves of rpd3^P-UTR/+ and rpd3^P-1.8/+ male and female flies exposed to 5% H₂O₂ at age 10 days of age. (H, K) Survival curves for male (H, J) and female (I, K) rpd3^def/yw, rpd3^def/dfoxo^c01841 and dfoxo^c01841/yw flies during starvation (H, I) or on 5% H₂O₂ (J, K) at age 40.