Research Paper Volume 8, Issue 11 pp 2948—2960

Figure 3. EWSAT1 is a direct target of miR-326/330-5p. (A) Screen of the candidate miRNAs that target EWSAT1 predicted by miRDB and PITA. (B) Sequence alignment of miR-326/330-5p with the putative binding sites within the wild-type regions of EWSAT1. (C-D) Detection of miR-326/330-5p using qRT-PCR in the sample pulled down by biotinylated EWSAT1 probe. (E-F) Up-regulated miR-326/330-5p in CNE-1 and SUNE-1 cells, which stably over-expressed EWSAT1, largely reversed the favorable effects of EWSAT1 on cell proliferation. Assays were performed in triplicate. *P< 0.05. Means ± SEM are shown. Statistical analysis was conducted using student t-test.