Figure 5. CLS assay of cells with overlong telomeres but no CDC13-EST2 fusion gene (A) CLS of normal- and overlong-telomere cells after plasmids eviction (21st streakout) (B) Southern blot analysis of telomere length using telomeric TG1-3 probe. (C) CLS of wild-type (4th streakout) and rif1∆ (4th streakout). (D) Telomere length detection by Southern blot at CLS D1 and D15.