Research Paper Volume 8, Issue 11 pp 2827—2847

Tethering telomerase to telomeres increases genome instability and promotes chronological aging in yeast

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Figure 4. Detection of CAN1 mutation frequency and GCR frequency in sch9∆ cells during chronological aging. (A) Cells of hxt13∆-pRS315/ hxt13sch9∆-pRS315 (streakout 14th, normal telomeres), hxt13∆-pRS315-CDC13-EST2/ hxt13sch9∆-pRS315-CDC13-EST2 (streakout 14th, overlong telomeres) were used to assay CAN1 marker-gene mutation frequency during chronological aging. (B) The same cells in (A) were used to examine GCRs frequency during chronological aging. The values (viable colonies) are the averages of 6-10 cultures ± SEM. (C) Telomere length of cells of CLS D1 and D29 in (A) and (B) was examined by Southern blot.