Research Paper Volume 8, Issue 11 pp 2814—2826

Figure 4. Caloric restriction preserves learning and long-term memory of aging mice. (A) An illustration of the Radial Arm Water Maze. Mice were trained to locate the hidden platform through a 2-day, 30 trials testing (3 blocks each day, 5 trials each block). Wrong entries were recorded as errors. (B) Young AL and CR mice did not have significantly different performances across the 6 blocks. (C) Old AL mice performed worse in Block 1 (initial learning phase) and Block 4 (initial recall phase) compared to the old CR mice. The comparison among the four groups for (D) Block 1 and (E) Block 4. Significant inverse correlation between errors made in Blocks 1 and 4 and CBF in (F) hippocampus (r² = 0.29, p < 0.001) and (G) frontal cortex (r² = 0.27, p < 0.001). Color codes indicate the four groups of mice. Data are mean ± SEM. **p < 0.01; AL: ad libitum; CR: caloric restriction.