Research Paper Volume 8, Issue 10 pp 2590—2602

Figure 7. Absence of global elevation of metabolic markers in brown fat in old Mtbp heterozygous mice. (A-H) Healthy Mtbp^+/+ (+/+; n=5; black) and Mtbp^+/- (+/-; n=8; gray) mice at 29 months were starved for 5 hours, sacrificed, and brown fat frozen. qRT-PCR for (A) glutaminase (Gls, p=0.4982), (B) hexokinase 2 (Hk2, p=0.4555), (C) ornithine decarboxylase (Odc, p=0.8677), (D) carbamoyl-phosphate synthetase 2/aspartate transcarbamylase/dihydroorotase, (Cad, p=0.9700), (E) nucleolin (Ncl, p=0.2668), (F) peroxisome proliferation activated receptor gamma coactivator 1-alpha (Pgc1α, p=0.0531), (G) Pgc1-beta (Pgc1β, p=0.8492, +/+ n=4 due to insufficient RNA), and (H) sirtuin-1 (Sirt1, p=0.4146) was performed. Values are relative to β-actin levels. P values calculated using student’s t-tests and error bars are SEM.