Research Paper Volume 8, Issue 10 pp 2590—2602

Figure 4. Long-lived Mtbp heterozygous mice exhibit normal systemic physiology. (A) The mean number of pups birthed from Mtbp^+/+ x Mtbp^+/- crosses by each female Mtbp^+/+ (+/+; n=20 females; 328 total pups; black) and Mtbp^+/- (+/-; n=25 females; 569 total pups; gray) mouse was recorded and averaged (p=0.2247). (B-H) Healthy long-lived (29 months) +/+ (n=5) and +/- (n=8) male mice were starved for 5 hours. (B) The nose-to-rump length was recorded and averaged (p=0.9999). (C) After sacrifice, the femurs were isolated and their length measured with electronic calipers and averaged (p=0.7160; n=7 for +/- group due to loss of one femur from bilateral fracture from collection). (D-H) Blood was collected and serum isolated. Circulating levels of (D) IGF-1 (p=0.4175), (E) cholesterol (p=0.3572), (F) triglycerides (p=0.4037), (G) blood glucose (p=0.7116) and (H) insulin (p=0.6963) were measured. P values calculated with student’s t-tests, and error bars are SEM.