Figure 4. (A) Representative immunohistochemical images (original magnification x400) of pmTOR expression in brain of long-term ad libitum- (AL, upper panel) and of caloric-restricted-fed (CR, lower panel) ApoE-/- mice. (B) Representative Western blot and densitometric analysis of pTau in brain of ApoE-/- mice. Mice were fed either AL or CR (60% of ad libitum) for a short-term (4 weeks; n=14), mid-term (20 weeks; n=14) or long-term (64 weeks; n=14). Signals were corrected to Tau. GAPDH served as loading control. Values are given as means ± SEM; ANOVA, post-hoc pairwise comparison tests: * p < 0.05 vs. AL.