Research Paper Volume 8, Issue 10 pp 2509—2524

Figure 6. Ectopic expression of miR-346 in A549 and SPC-A-1 cells reduces cell migration and invasion motility. (A-D) A549 and SPC-A-1 cells were loaded onto the top well of a transwell inserts for cell migration or invasion assay. After twenty four hours, cells that migrated to the bottom chamber containing serum-supplemented medium were stained with 0.1% crystal violet, visualized under a phase-contrast microscope, and photographed. Total number of cells in five fields was counted manually. Assays were performed in triplicate. *P < 0.001, Means ± SEM was shown. Statistical analysis was conducted using student t-test.