Research Paper Volume 8, Issue 10 pp 2463—2487

Figure 7. PRG3 impedes non-hydrolysable lysophosphatidic acid (LPA) induced axon collapse. (A) Representative time laps microscopy image series of GFP and PRG3 expressing neurons treated with 0.5 µM nhLPA (non-hydrolysable lysophosphatidic acid, which is resistant to any ecto-phosphatase activity). Transfection efficacy is shown in first image bottom right corner. Scale bar represents 50 µm. (B) Quantification of neurite length of neurons expressing GFP (control) or PRG3. Note, that PRG3 expressing neurons are also resistant to the nhLPA collapse inducing activity. Three independent experiments were carried out and differences were considered statistically significant with * p<0.05, ** p<0.01, *** p<0.001 (two-tailed t-test). Values are given as mean ± SEM.