Research Paper Volume 8, Issue 9 pp 2100—2126

Figure 3. DAF-16 and SKN-1 are required for longevity from reduced cell cycle activity. (A) Lifespan extension in response to cell cycle inhibition is mediated by DAF-16. daf-16(mgDf47) null mutants and wild type worms were fed with cye-1 and control(RNAi). The longevity associated with cye-1(RNAi) was greatly decreased but not eliminated by daf-16 mutation. (B) Longevity of cye-1(RNAi) was greatly reduced by kri-1(ok1251) mutants. (C) Knockdown of cye-1 by RNAi further increases longevity of daf-2(e1370) mutants. (D) Longevity extension by cye-1(RNAi) was eliminated by skn-1(zu67) mutation. Survival plots show combined data from at least two experiments and are summarized in Table 1, individual experiments are presented in Table S1. EV refers to empty control vector.