Research Paper Volume 8, Issue 10 pp 2355—2369
Caveolin-1 controls mitochondrial function through regulation of m-AAA mitochondrial protease
Figure 1. AFG3L2 directly interacts with caveolin-1 in vitro. (A) Schematic diagram showing the consensus caveolin-binding-domain (CBD) and the CBD of human AFG3L2 (amino acids 138-146 and 147-154). Φ represents an aromatic amino acid and X represents any amino acid. (B) Caveolin-1-GST fusion proteins [GST-Cav-1(82-101), GST-Cav-1(1-101) and GST-Cav-1-FL] were used in pull down assays with cell lysates from NIH 3T3 cells transiently transfected with AFG3L2-myc. Pull-down assays with GST alone was used as internal control.