Research Paper Volume 8, Issue 10 pp 2337—2354
TGF-β and NF-κB signaling pathway crosstalk potentiates corneal epithelial senescence through an RNA stress response
Figure 5. Effects of TGF-β1 on HCECs RNA SGs and IκBα mRNA decay. (A-B) mRNA levels of SG and PB components (A) or HuR immunostaining (B) in HCECs treated with TGF-β1 (10 ng/ml) for 24h alone, or in combination with indicated siRNA. Nuclear staining by DAPI revealed cells in sections. Images show representative HuR-containing aggregates. (C-D) Phosphorylation of eIF2α was detected by western blot after TGF-β1 (10 ng/ml) treatment for 24h. (E) mRNA levels of IκBα in HCECs treated with TGF-β1 (10 ng/ml) alone, or in combination with LY364947 (2μM) for 24h. (F-G) IκBα mRNA levels in HCECs treated with TGF-β1 (10 ng/ml) (F) or actinomycin D (AcD) alone or AcD together with TGF-β1 (G) for the indicated durations. **P≤0.01,*P ≤0.05.