Research Paper Volume 8, Issue 10 pp 2337—2354
TGF-β and NF-κB signaling pathway crosstalk potentiates corneal epithelial senescence through an RNA stress response
Figure 1. Senescence associated markers rise with age in human corneal epithelium. (A) The mRNA expression of p16 and p21 in young donors(≤30 years of age) and old donors (≥50 years of age) corneal epithelium(**P≤0.01, n=3).(B) Immunoblot analysis of p16 and p21 in the corneal epithelium during aging. (C) Quantification of western blots processed with Image J. (D-E) Representative photographs (D) and histopathology scores (E) for the IHC staining of p16 and p21 in corneal epithelium from donors of different ages. There were statistically significant differences in p16 and p21 expression between the donors of younger than 30 years and older than 50years of age (P≤0.05). The figure depicts a Pearson correlation of p16 and p21 protein expression with age (E).