Research Paper Volume 8, Issue 9 pp 2006—2022

Figure 6. SATB2 binds to human Nanog promoter and promotes its transcription in AB-BMSCs. The mRNA levels of Nanog were determined by real-time PCR upon SATB2 overexpression (A) and knockdown (B) in AB-BMSCS. (C) Twelve primers were designed to cover the human Nanog promoter region and used to identify binding sites of SATB2 by ChIP assay. Four putative SATB2-binding sites in Nanog promoter region were identified. The relative enrichment of these four putative binding sites of SATB2 in AB-BMSCs from young (D) and older donors (E). (F) Luciferase reporter assays indicated that the luciferase activity was significantly increased in reporter containing the binding site 10. (G) Schematic diagram illustrating the roles of SATB2-Nanog axis in aging-related properties of AB-BMSCs. #p> 0.05, *p< 0.05, **p< 0.01.