Research Paper Volume 8, Issue 8 pp 1703—1717
Paracrine effects of human adipose-derived mesenchymal stem cells in inflammatory stress-induced senescence features of osteoarthritic chondrocytes
Figure 10. Effect of Sirt1 siRNA on the reduction of γH2AX foci accumulation by CM. (A) Representative experiment showing that Sirt1 siRNA (siSirt1) inhibits the effect of CM on the accumulation of γH2AX (red pixels) positive cells induced by IL-1β. siCont: non-specific siRNA. DAPI was used to stain the nuclei. Bars: 50μm. (B) Percentage of positive cells. (C) Representative experiment showing that Sirt1 siRNA (siSirt1) inhibits the effect of CM on the accumulation of γH2AX (red pixels) foci per cell induced by IL-1β. siCont: non-specific siRNA. DAPI was used to stain the nuclei. Bars: 5μm. (D) Number of γH2AX foci per cell. OA chondrocytes were incubated with the indicated treatments for 3 days. Data are shown as mean±standard deviation of N=4 separate experiments with cells from separate donors. ++p<0.01 with respect to non-treated cells; *p<0.05 with respect to IL-1β-treated cells; #p<0.05, ##p<0.01 with respect to IL-1β+CM.