Research Paper Volume 8, Issue 7 pp 1442—1456
miR-638 suppresses DNA damage repair by targeting SMC1A expression in terminally differentiated cells
Figure 8. The overexpression of miR-638 affects γH2AX protein expression and foci formation. (A) K562 cells were transfected with mimics-NC or mimics-638 and then exposed to cisplatin (25 μM) for 30 min. The protein level of γH2AX was assessed via western blotting. β-actin served as the loading control. (B) The same treated K562 cells in (A) were collected to analyze γH2AX (red) localization in the cell nucleus by immunofluorescence microscopy. The nucleus was visualized by DAPI (blue) staining. (C) The number of cells with γH2AX foci formation was counted, and the statistical number of each group was shown below (n). NS: not significant, *: p< 0.05, ***: p<0.001, compared to control with the Student's t test. Error bars, S.D.