Research Paper Volume 8, Issue 7 pp 1470—1484

Figure 5. Ghrelin mediates caloric restriction-induced autophagy in rat cortical neurons. Primary rat cortical neurons were treated with GHS-R1a receptor antagonist [D-Lys³]-GHRP-6 (GHS-R1a ant, 100 μM) 30 min before caloric restriction (CR) for 6 h. Whole cell extracts were assayed for LC3B-II (A), SQSTM1 (B) and β-tubulin (loading control) immunoreactivity through Western blotting analysis, as described in Materials and Methods. The results represent the mean ± SEM of, at least, five independents experiments, and are expressed as percentage of control. *p<0.05 and ***p<0.001, significantly different compared to control; ^#p<0.05, ^##p<0.01 and ^###p<0.001, significantly different from caloric restriction, as determined by ANOVA, followed Bonferroni’s post test.