Research Paper Volume 8, Issue 7 pp 1470—1484

Figure 2. Caloric restriction increases NPY and ghrelin levels in rat cortical neurons. Primary rat cortical neurons were exposed to caloric restriction medium (CR), DMEM low glucose, for 6 h. Cells in neurobasal medium with 2% B27 supplement were used as control (Ctrl). (A and B) Total RNA was isolated and the transcript levels of NPY and ghrelin were analyzed by qRT-PCR, as described in Materials and Methods. The results represent the mean ± SEM of five independents experiments and are expressed as the relative amount compared to control. **p<0.01 and ***p<0.001, significantly different compared to control, as determined by Student’s t test. (C and D) Caloric restriction increased NPY and ghrelin protein content, determined by Enzyme-Linked Immunosorbent Assays, as described in Material and Methods. The results represent the mean ± SEM of 3-4 independents experiments and are expressed as the relative amount compared to control. **p<0.01 significantly different compared to control, as determined by Student’s t test.