Research Paper Volume 8, Issue 7 pp 1540—1570

Figure 8. Cyclin A2 loss impairs hippocampal development. (A) Cavalieri estimations of the size of the dentate gyrus were performed in CCNA2^fl/fl, Nestin-cre brains at P0 or P14, and in CCNA2^fl/fl, CamkIIa-cre brains at 4 months or 8 months of age. The y-axis is volume of the dentate gyrus. Age and cre-driver are indicated below. Unpaired t-test, * = p < 0.05, n.s. = not significant, n=3 animals per condition for P0, P14, and 4 months; n=4 animals per condition for 8 month animals. (B, D) Cryosections of brains were stained for γH2AX as in Fig. 7 and foci counted in the dentate gyrus (B) or CA1 layer in 50x40 μm counting frames (D). White arrows represent γH2AX foci in the nucleus, yellow arrows represent background staining that was not counted. (C, E) Quantification of γH2AX in the dentate gyrus (C) or CA1 layer (E). The y-axis is γH2AX foci per counting frame. ANOVA with Tukey’s HSD, * = p < 0.05. n = 3 animals per experimental condition. Error bars represent s.e.m.