Research Paper Volume 8, Issue 7 pp 1540—1570

Figure 7. Rad51 levels are increased in the VZ/SVZ of CCNA2^fl/fl, Nestin-cre animals. (A) Cryosections from E14.5 brains were stained for Rad51. The VZ/SVZ is denoted by a dashed line. (B) Quantification of Rad51 levels in the E14.5 VZ/SVZ shown in (A). Profile plots of fluorescence intensity in 10 μm bins from the ventricle show increased Rad51 levels in the VZ/SVZ. The y-axis is Rad51 fluorescence intensity, and the x-axis is distance from the ventricle. Unpaired t-test, * = p < 0.05, n=3 animals per condition. (C) Sections of E14.5 brains were stained for γH2AX. Arrows indicate foci of γH2AX. (D) Quantification of γH2AX levels in the E14.5 VZ/SVZ shown in (C). γH2AX foci are decreased in CCNA2^fl/fl, Nestin-cre animals compared to controls. γH2AX foci were counted in a standard 50x90 μm counting frame. The y-axis is γH2AX foci per counting frame. Unpaired t-test, * = p < 0.05, n=3 animals per condition. (E) Cryosections of P0 brains were stained for Rad51. Arrows indicate Rad51-positive cells. The VZ/SVZ is denoted by a dashed line. (F) Quantifications of images from (E). Percentage of cells expressing Rad51 were counted and normalized to total nuclei in the VZ/SVZ. The percentage of Rad51-positive cells is increased in CCNA2^fl/fl, Nestin-cre brains. The y-axis is the percentage of cells in the VZ/SVZ that express RAD51. Unpaired t-test, * = p < 0.05, n=2 animals per condition. Error bars for all graphs represent s.e.m.