Research Paper Volume 8, Issue 8 pp 1650—1669
Ionizing radiation-mediated premature senescence and paracrine interactions with cancer cells enhance the expression of syndecan 1 in human breast stromal fibroblasts: the role of TGF-β
Figure 8. Involvement of the TGF-β signaling pathway in SDC1 overexpression by senescent human breast stromal fibroblasts. The levels of the SDC1 gene expression were assessed by real-time RT-PCR in ionizing radiation-mediated senescent due to irradiation (IS) breast stromal fibroblasts, after treatment in the presence or absence of the TGFβR1 inhibitor SB431524 or the Sp1 inhibitor mithramycin (MTR) (A) or after silencing of Smad4 expression by siRNA (B). One representative experiment out of three similar ones is depicted (* indicates p <0.05 in comparison to the control).