Research Paper Volume 8, Issue 8 pp 1650—1669

Figure 6. Signaling molecules responsible for SDC1 upregulation by TGF-β in human breast stromal fibroblasts. The induction of the SDC1 gene expression by TGF-β in young human breast stromal fibroblasts was assessed by real-time RT-PCR in the presence or absence of the TGFβR1 inhibitor SB431524 or the Sp1 inhibitor mithramycin (A) or after silencing of Smad4 expression by siRNA (B); representative experiments out of three similar ones are presented (* indicates p < 0.05 in comparison to the untreated control). In (C) the efficiency of Smad4 silencing was assessed by western blotting (one representative experiment is depicted).