Research Paper Volume 8, Issue 8 pp 1650—1669

Figure 5. Effect of exogenous and paracrine growth factors on SDC1 expression in human breast stromal fibroblasts. (A) Early-passage human breast stromal fibroblasts were treated with PDGF (10 ng/ml), FGF2 (10 ng/ml), EGF (100 ng/ml), IGF-I (100 ng/ml) or TGF-β (5 ng/ml) and after 24 h SDC1 expression was assessed by RT-PCR. (B) Cells were pre-incubated for 1 h with growth factor-receptor inhibitors [STI571 for PDGFR (2 μM); SU5402 for FGFR1 (20 μM); AG1478 for EGFR (3 μM); I-OMe-AG538 for IGFIR (12 μM); SB431542 for TGFβR1 (10 μM)]. Then, cells were treated with medium conditioned by MDA-MB-231 (50% v/v), and 24 h later SDC1 expression was estimated by RT-PCR. One representative experiment out of three similar ones is depicted (* indicates p < 0.05 in comparison to the untreated control).