Research Paper Volume 8, Issue 8 pp 1650—1669

Figure 3. SDC1 expression in senescent human breast stromal fibroblasts in vitro and in vivo. In (A) the expression of the SDC1 gene in young (Y) and senescent due to irradiation (IS) breast fibroblasts was assessed by real-time RT-PCR; mean values (± standard deviation) of three independent experiments are presented (* indicates p < 0.05 compared to Y cells). In (B) the protein expression of SDC1 on the surface of Y and IS cells was studied after recognition with a specific antibody and flow cytometric analysis (one representative experiment out of three similar ones is presented), while in (C) SDC1 was immunolocalized in tissue sections from irradiated vs. non-irradiated (control) human breast tissue from the same individual. Finally, in (D) cells stained histochemically with Sudan Black B (SBB positive black granules - arrows) and immunohistochemically for Sdc1 (brown color - arrowheads) in irradiated human breast tissue are depicted (Magnification: images x630; inserts x1000).