Research Paper Volume 8, Issue 8 pp 1650—1669
Ionizing radiation-mediated premature senescence and paracrine interactions with cancer cells enhance the expression of syndecan 1 in human breast stromal fibroblasts: the role of TGF-β
Figure 2. The catabolic phenotype of senescent human breast stromal fibroblasts. In (A) the expression of genes related to ECM turnover was studied by real-time RT-PCR in senescent due to irradiation (IS) fibroblasts; the expression of each gene is presented relative to its expression in young (Y) cells. In (B) MMP activity was measured in the conditioned media of Y vs. IS cells using the TNO211 substrate, while in (C) the collagen synthesized by Y and IS fibroblasts was assessed after [3H]-proline labeling by the protease-free collagenase method. One representative experiment out of three similar ones is depicted; asterisks indicate statistically significant differences in comparison to Y cells (p < 0.05).