Research Paper Volume 8, Issue 8 pp 1650—1669

Figure 1. Characterization of irradiation-induced premature senescence in human breast stromal fibroblasts in vitro and in vivo. Early passage (young: Y) and senescent due to irradiation (IS) cells were grown in vitro under standard conditions in the presence of 10% (v/v) FBS. In (A) cells were stained for SA-β-gal or for BrdU incorporation, while DAPI staining was used for nuclei identification. In (B) cell lysates from young and senescent cells were analyzed by western blot for the expression of the indicated proteins. In (C) Sudan Black B staining of tissue sections from irradiated vs. non-irradiated (control) human breast tissue from the same individual was performed. One representative experiment out of three similar ones is depicted.