Research Paper Volume 8, Issue 7 pp 1330—1349

Figure 3. Mitophagy deficiency impairs the bioenergetic transition associated with nuclear reprogramming. (A) Calculated glycolysis (maximum rate measurement before oligomycin injection – Last rate measurement before glucose injection [1]), glycolytic capacity (maximum rate measurement after oligomycin injection – Last rate measurement before glucose injection [2]), and glycolytic reserve (glycolytic capacity – glycolysis [3]) were calculated with normalized ECAR values in PINK1^+/+- and PINK1^−/−-iPSCs (n = 2). (B) Two-dimensional PLS-DA models to view the separation of the two groups (PINK1^+/+- and PINK1^−/−-iPSCs) following GC-EI-QTOF-MS-based metabolomic profiling. (C) Metabolites in PINK1^+/+-iPSCs were extracted and quantitatively analyzed by GC-EI-QTOF-MS and compared with metabolites from PINK^−/−-iPSCs (n = 2). Significantly increased and decreased metabolites are shown using yellow-red and light blue-dark blue color scales, respectively (see also Table 1).