Research Paper Volume 8, Issue 7 pp 1330—1349

Figure 2. Mitophagy deficiency impedes the rejuvenation of mitochondria networks in iPSCs. (A) Loss of PINK1 induces moderate mitochondrial fragmentation and aggregation in MEFs (MT: MitoTracker). Bar chart depicts the average percentages of cells showing fragmentation and/or aggregation (n=3). *, P<0.05. (B) Representative TEM images of mitochondria in all cell lines. PINK1^+/+ and PINK1^−/− MEFs display a preponderance of tubular and cristae-rich mature mitochondria. PINK1^+/+ iPSCs display a preponderance of “rejuvenated” spherical cristae-poor immature mitochondria, whereas PINK1^−/− iPSCs display an impaired “rejuvenation” characterized by an assortment of mitochondrial configurations including round (immature) and more enlarged mitochondria with disorganized cristae