SIRT6 protects against endothelial dysfunction and atherosclerosis in mice

Suowen Xu; Meimei Yin; Marina Koroleva; Michael A. A. Mastrangelo; Wenbo Zhang; Peter Bai; Peter J. J. Little; Zheng Gen Gen Jin

doi:doi:10.18632/aging.100975

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Research Paper Volume 8, Issue 5 pp 1064—1078

SIRT6 protects against endothelial dysfunction and atherosclerosis in mice

Figure 2. Endothelium-specific deletion of SIRT6 impairs endothelium-dependent relaxation. (A) Schematic diagram of the transgenic mice used to generate endothelium-specific SIRT6 deficient (ecSIRT6^−/−) mice and control SIRT6 wild-type (ecSIRT6^+/+) mice. (B) Intimal endothelial cell lysates were harvested from ecSIRT6^+/+ and ecSIRT6^−/− aortae and analyzed for the deletion of SIRT6 by Western blotting, n=4. (C) Reactivity of ecSIRT6^+/+ and ecSIRT6^−/− aortic rings to acetylcholine (Ach), ***P<0.001, compared to ecSirt6+/+ littermates, n = 9; (D) Reactivity of ecSIRT6^+/+ and ecSIRT6^−/− aortic rings to sodium nitroprusside (SNP), n = 10.