Figure 2.Endothelium-specific deletion of SIRT6 impairs endothelium-dependent relaxation(A) Schematic diagram of the transgenic mice used to generate endothelium-specific SIRT6 deficient (ecSIRT6−/−) mice and control SIRT6 wild-type (ecSIRT6+/+) mice. (B) Intimal endothelial cell lysates were harvested from ecSIRT6+/+ and ecSIRT6−/− aortae and analyzed for the deletion of SIRT6 by Western blotting, n=4. (C) Reactivity of ecSIRT6+/+ and ecSIRT6−/− aortic rings to acetylcholine (Ach), ***P<0.001, compared to ecSirt6+/+ littermates, n = 9; (D) Reactivity of ecSIRT6+/+ and ecSIRT6−/− aortic rings to sodium nitroprusside (SNP), n = 10.
