Figure 3.Depletion of MAP1S causes accumulation of fibronectin and renal fibrosis in aged mice(A) Immunostaining analyses of fibronectin (green) in sections from renal tissues renal tissues collected from wild-type and MAP1S−/− mice at different ages using anti-fibronectin antibody. Nuclear DNA is counter-stained as red. Bar: 50 μm. (B-E) Representative immunoblot images (B) and plots (C-E) showing the impacts of MAP1S on the levels of fibronectin (C), TGF-β (D) and α-SMA (E) in renal tissues described in panel (A). The initial intensity of each protein in the 12-month-old wild-type was set to be 1. Data shown in plots above were the averages and standard deviations of three repeats. Plots were the means ± S.D. of three repeats and the significance of the differences was compared as described above. (F) Comparative Sirius Red staining of renal tissues described in panel (A). Bar = 100 μm.
