Research Paper Volume 8, Issue 5 pp 977—985

Figure 3. Depletion of MAP1S causes accumulation of fibronectin and renal fibrosis in aged mice. (A) Immunostaining analyses of fibronectin (green) in sections from renal tissues renal tissues collected from wild-type and MAP1S^−/− mice at different ages using anti-fibronectin antibody. Nuclear DNA is counter-stained as red. Bar: 50 μm. (B-E) Representative immunoblot images (B) and plots (C-E) showing the impacts of MAP1S on the levels of fibronectin (C), TGF-β (D) and α-SMA (E) in renal tissues described in panel (A). The initial intensity of each protein in the 12-month-old wild-type was set to be 1. Data shown in plots above were the averages and standard deviations of three repeats. Plots were the means ± S.D. of three repeats and the significance of the differences was compared as described above. (F) Comparative Sirius Red staining of renal tissues described in panel (A). Bar = 100 μm.