Ischemic stroke induces gut permeability and enhances bacterial translocation leading to sepsis in aged mice

Joshua Crapser; Rodney Ritzel; Rajkumar Verma; Venugopal R. R. Venna; Fudong Liu; Anjali Chauhan; Edward Koellhoffer; Anita Patel; Austin Ricker; Kendra Maas; Joerg Graf; Louise D. D. McCullough

doi:doi:10.18632/aging.100952

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Research Paper Volume 8, Issue 5 pp 1049—1060

Ischemic stroke induces gut permeability and enhances bacterial translocation leading to sepsis in aged mice

Figure 4. The effects of age and stroke on peripheral lymphocytes 72 hours after 90 minute MCAO. (A) There was an effect of stroke (F(1,46)=p<0.0001) on the ratio of circulating lymphocytes to myeloid cells, a measure of lymphopenia, as analyzed by 2-way ANOVA (n=10-15/group). This ratio was significantly reduced in young (p<0.05) and aged (p<0.0001) mice following stroke. (B) There was a significant effect of stroke (F(1,14)=p<0.005, n=3-6/group) but not age on the proportion of circulating CD69⁺ CD4⁺ T cells as analyzed by 2-way ANOVA. (C) The proportion of circulating CD8⁺ T cells expressing CD69 was affected by both stroke (F(1,14)=p=0.05) and age (F(1,14)=p<0.05) via 2-way ANOVA (n=3-6/group). Values are expressed as mean ± SEM. *, p≤0.05; ***, p<0.001.