Research Paper Volume 8, Issue 5 pp 933—940
MMP-9 overexpression is associated with intragenic hypermethylation of MMP9 gene in melanoma
Figure 4. Correlation of MMP-9 CpG-2 hotspot with MMP-9 expression in melanoma cell lines. (A) RT-qPCR analysis of MMP-9 transcription levels. (B) ELISA determination of released MMP-9 in cell medium supernatant. (C) Methylation analysis of MMP9 CpG-2 hotspot in melanoma cell lines by MSRE assay. One-way ANOVA test was performed to analyze statistical significance.