Figure 4. Characterization of the derived hiPSCs. (A) Cell morphology and immunocytochemistry analysis of pluripotent markers OCT4 and TRA-1-60 in a representative control hiPSC line (MRU11780) and LHON hiPSC line (LHON Q1-4). Scale bar = 100μm. (B) In vitro embryoid body differentiation of hiPSC into cell representative of the endoderm (AFP), mesoderm (SMA) and ectoderm (NESTIN). Scale bar = 100μm. (C) hiPSCs form teratoma containing endodermal cells (e, gut-like epithelium), mesodermal cells (c, cartilaginous structure; a, adipose tissue) and ectodermal cells (nr, neural rosette). Scale bar = 50μm.