Research Paper Volume 8, Issue 5 pp 945—957
Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells
Figure 3. Reprogramming of control and LHON fibroblasts using feeder-free system. Representative pictures of (A) TRA-1-60 positive colony and (B) TRA-1-60 negative colony. (C) Quantification of hiPSC colonies generated from control and LHON fibroblasts. Blue bar indicates bona fide hiPSC colonies, as defined by TRA-1-60 expression and hESC-like morphology, per 50,000 reprogrammed cells. Red bar indicates partially reprogrammed colonies as defined by absence of TRA-1-60 expression and/or resemble non-hESC-like morphology, per 50,000 reprogrammed cells. n=3, error bars represent SEM. (D) Pooled results of hiPSC colony number generated from control and LHON fibroblasts. ns = not significant, as indicated by unpaired t-test.