Research Paper Volume 8, Issue 5 pp 945—957
Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells
Figure 2. OXPHOS analysis of control and LHON fibroblasts. (A) Oxygen consumption of control and LHON fibroblasts, showing the endogenous respiration rate (Endog), ADP-stimulated cell respiration with glutamate + malate (CxI-ADP) or with succinate (CxI+II-ADP), the uncoupled maximal respiration by addition of CCCP (CxI+II-UC) and the complex II respiration with uncoupled feedback by addition of rotenone (CxII-U). n = 3 for each patient fibroblast. Error bars represent SEM. (B) Complex I respiration of individual fibroblast samples and pooled data, normalized to uncoupled maximal respiration (CxI-ADP/CxI+II-UC). (C) Uncoupled complex II respiration (CxII-U/CxI+II-UC) in control (CERA007, MRU11780, BJ) and LHON fibroblasts (LHON V31-1, LHON T1-20, LHON Q1-4). **** = p<0.0001, *** = p<0.001, ** = p<0.01, * = p<0.05, ns = not significant.