Research Paper Volume 8, Issue 4 pp 810—830
Mitotic degradation of yeast Fkh1 by the Anaphase Promoting Complex is required for normal longevity, genomic stability and stress resistance
Figure 7. The Fkh1K-R mutation does not impair recruitment of Fkh1 to CLB2 and FKH2 promoters. (A) ChIP was performed on the strains shown. DNA was purified and used as template in PCR reactions using primers specific for the loci indicated. 36-38 cycles were determined to be in the linear range for these PCR reactions. DNA extracted prior to IP was used as input controls. (B) The DNA bands in (A) were scanned, quantified, and a ratio of bound:input DNA was plotted.