Research Paper Volume 8, Issue 4 pp 810—830

Figure 3. APC subunit content controls Fkh1 protein levels. (A) Fkh1-TAP cells expressing plasmid-borne GST-APC5, or the empty vector control, were grown to early log and then exposed to 50 μM CuSO₄ for 0, 1 or 2 hours. Cell lysates were prepared and analyzed using Westerns with antibodies against TAP, GST and GAPDH as a load control. (B) The same experiment as in (A) was performed except the plasmid expressed GST-APC10. (C) The bands from 3 repeat experiments in (A) were scanned, quantified, normalized to GAPDH, and plotted. (D) The bands from 2 repeat experiments in (B) were scanned and treated as in (C). Standard error of the mean is shown. Significance was calculated using a Student t-test.