Research Paper Volume 8, Issue 4 pp 810—830

Figure 1. Deletion of FKH1 reverses APC mutant phenotypes. (A) The various yeast strains shown were grown overnight at 30°C. The next morning a 10-fold serial dilution series starting with 1 × 10⁷ cells/ml was spotted onto YPD plates and grown at 30°C and 37°C for 3 to 5 days. (B) The strains shown were treated as above. (C) Cells were spot diluted and grown at 30°C and 40°C to accentuate temperature sensitive growth. (D) The cells shown were grown to day 5 of stationary phase then split, with one half treated with 100 mM H₂O₂ for 1 hour. Equal numbers of cells were then plated onto YDP with all treated cell counts compared to untreated controls. The experiment was done in triplicate with standard error shown. (E) Replicative lifespan was performed with the cells shown. (F) Chronological lifespan of the cells used in (E) was performed in triplicate. Cells were grown in Complete Media (CM) and maintained in the Depleted Media (DM) once stationary phase was reached. Standard error of each timepoint is shown.