Figure 1. Orthogonal signal correction partial least squares discriminant analysis (O-PLS-DA) demonstrating the effect of graded CR on gene expression levels of aging-associated genes. 24AL, 12AL, 10CR, 20CR, 30CR and 40CR refer respectively to 24h ad libitum feeding per day, 12h ad libitum feeding per day, 10 %, 20 %, 30 % and 40 % restriction.